Prescience's buff now makes it work on all charge levels, which really helps on the charge weapons like Rifles, Spears and ClubsĪlso most charge attacks for weapons have increased close to, or more than double in damage. Mitama build potential is only slightly factored in this.ĭamage value changes i have not been able to find for Rifles and Bows, so this hardly influenced this order. Example: Naginata's were the worst damage weapon in kiwami, Having the 2nd most buffs doesn't mean its the 2nd strongest weapon now). Please note this isn't a tier list on what are the best weapons (Like this doesn't mean the bottom list will be the strongest and/or best weapon. These are in order of biggest nerfs to biggest buffs. Old Weapon changes (+Good change, -Bad change, ~Misc or netural change) (These are a special set of weapons that require a specific weapon and mitama to make) I have posted a list of the Toukiden 2's Forge weapons further down this thread to show examples of what kind of weapon stats to expect. 3 endgame Elemental based weapons in a weapon tree have a Crit Rate of 45% almost exactly the same rate of the best Weapons for a Pure Critical Build. So a Rank 6 Weapon will be considered endgameĪn Example. Base Crit Damage is still 150%Īnother change is Critical damage also now affects your Elemental Damage opening up many possibilities for hybrid setups mixing Crit and Elemental Damageīase stats of weapons have received a bit of a change looking very different when comparing to similiar weapons from Toukiden Kiwami. The Devastation ability now only adds 20% onto Crit Damage. This new formula means that adding mitama abilities that increase your damage like Devotion or Knowledge will actually lower your Critical Rate so keep that in mind if trying to focus on builds involving a high Critical Rate ( ( Dexterity x 3 ) / ( Attack + Elemental Attack ) x 100 ) The new Critical Rate Formula is as followed. The reason why the Critical related stat in Toukiden 2 was changed from Precision to Dexterity is because the Formula for determining your Critical Rate has changed. Growing your weapons from 9-12 seem to be different and possibly set in stone on what grows but the stat increase is much larger. Normally Equipment can only go up to Level 9.Īfter beating the game's story campagin a Special NPC named Kiyomaro can be sometimes be found in the Village or Safe Zone checkpoints in the open world. The only safe way to look at their stats is if the information posted showed their stats of the weapons unupgraded. This new Weapon leveling system sadly means looking up weapons' stats on guides or spreadsheets can be a problem. Say if you had 2 different Spears with near identical stats and you leveled them both up all in Dexterity, one may have a 10% Crit Rate increase while the other 20. Some weapons grow differently than others if they were leveld up in the same stats. Giving many possibilities and having a purpose to making copies.įor each level up you can upgrade in Attack, Dexterity (critical rate), or Elemental damage or Ailment affliction (if the weapon allows it, you cannot add or remove a element or ailment to a weapon). You also have a choice on how you want your weapon to grow. Instead of gaining experience by using the weapon, you are using specific materials to level up your weapon. Leveling weapons are much different in Toukiden 2. And a brief look at some endgame weapons including a look at the Forge weapon list and which ones are good or not.The weapon-specific abilities to make them better, their changes and which mitama have them.Changed things such as how weapons level up now and changes in Critical Rate/Damage.Showing footage of the game's weapon tutorials.Since all of this information is so massive, it will be divided into several threads. Since translating can be a bit of a pain and no first-hand experience with the game itself, this information is not 100% accurate so there will be some incorrect information or translation errors when comparing with the future full release. This information was gathered from translating Japanese wikis and videos on a weekly basis over the course of 9 months since the game's initial Playstation Japanese release. Giving a massive amount of information and commentary to build hype or planning for those wanting to get into Toukiden 2. Greetings everyone, I'm JK3, local Toukiden guru, and this is one part of my Pre-Release Info dump series.
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You'll mostly be using the Veil of Mist brush technique to get through quasi-puzzles in the dungeons, however it can be effective in combat. Not all enemies are vulnerable to Inferno, but those that are feel it bad. When fighting enemies that have flames, you can use Inferno to bring the flame to other enemies. The places where you'll need to use Inferno for puzzle solving should be obvious, but chances to use it in combat may not be so apparent. Inferno works for both puzzle solving and combat. Some enemies (including the black imp) can be completely knocked out of the air, making them easier to combat. In combat, use the Galestorm brush technique to affect flying enemies. The direct you draw the line determines the direction of the wind, so if your first try doesn't yield the desired result the try drawing the opposite direction. This brush technique is useful both in puzzle solving and in combat. This is useful when you want to explore an area at night, as you might when hunting down wanted monsters to cross off your list. Use it to turn day to night or to simply reset the nighttime clock. The Crescent brush technique has the opposite effect of the Sunrise technique, as you can imagine. Draw a straight line going upwards from bubbling power spring to create a fountain platform which you can ride, letting you reach ledges above. You can use it to put out fires, including fires that cover item chests, giving you access to their goods. Watersprout can be used in a couple of different ways. You may need to quickly find another blossom to swing to. When swinging to upside-down Konohana blossoms, be prepared to fall after the swing. Make sure you see the green Holy Smoke before starting and ending your brush stroke to ensure the Vine catches its target. Connect either Amaterasu or large hooks to Konohana blossoms to create the vine. There are only a couple of uses for the Vine technique, but it's important to know how it works. Draw a Water Lily just below a ledge to create a platform from which you can jump high. In addition to helping you swim further, Water Lily can be used to let you reach ledges that overhang bodies of water. By stepping onto a Water Lily, you'll reset Amaterasu's breath meter, letting you continue to swim further. You'll need to use the Water Lily brush technique in a few areas in order to traverse over large bodies of water. If you swing the camera around, you can often line up multiple trees to revive with just one Bloom stroke. Use it often to revive wilted trees and earn valuable Praise needed to increase your powers. Use this knowledge to better position your Cherry Bombs.īloom is one of the most important brush techniques. drawing the bomb big on the screen) will create a Cherry Bomb close to the screen. When drawing a Cherry Bomb, remember that a smaller brush stroke will place the bomb further away from you while a large brush stroke (i.e. When the Cherry Bomb is drawn next to objects that can be exploded it will detonate almost instantly, making it easier to hit moving enemies with the attack. The Cherry Bomb can be used to blow up environment obstacles (look for cracks in the walls) as well as enemies. Another use for Power Slash: Use the brush technique to open underwater chests and release their items. Position the camera as you ready your brush to slash through everything at once. Remember that your slash can hit multiple enemies and items with just one stroke. You can also use Power Slash to knock items from trees and break pots. In combat, use Power Slash to break through enemy defenses (though not all enemies are always vulnerable-check out the Basics section of this guide for details on fighting enemies). Power Slash is useful in many situations, including combat and in general exploration. One thing you may not know: Press down the TRIANGLE button firmly to make a wide stroke that makes it easier to cover empty space. It's very simple and straight-forward-simply use Rejuvenation to fill in gaps in bridges and repair other obviously damaged things. This is one of the least used brush techniques in the game. When we're exploring, we use the Sunrise brush technique frequently to make sure we don't get interrupted with a brief scene showing the falling of night. You can use this to change night into day, or to simply reset the game's clock to avoid a transition to night. Draw a circle in the sky and the sun will come out. 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While somewhat expensive, you can literally craft Forgotten Souls thanks to Kanai's Cube. Five more areas have been added to this guide for more valid options. While somewhat expensive, you can literally craft Forgotten Souls thanks to Kanai's Cube. At this point in the game, experience and gold are worthless, as are most items so players need to have a clear goal in mind when they farm. To farm efficiently, players need to have the right gear and skills, but the most important thing to have is an idea of where to farm. When it comes to veterans and pros, most of the game is all about farming, not just winning. Updated on December 22nd, 2021 by Hodey Johns: While many gamers are just looking for a way to beat the game once, those who manage to crawl through Normal conquer Hell difficulties will be looking for something else. The good news is that over the years, the community has generally agreed on the best farming and grinding spots in Diablo 2: Resurrected so they can get the stuff they covet. In any other medium, that would be the definition of insanity but this is the action RPG genre and the players love their item roulettes and piñatas. RELATED: Diablo 2: Resurrected - How To Build The Wind Druid And how do they become powerful? By doing the same things over and over again. So how do players go about that task? By becoming powerful. Players of the original console Diablo III game on PlayStation 3 and Xbox 360 that caps out at level 60, or PC players who do not have the expansion, should instead look in the older Patch 1.0.8 Data Archive.In Diablo 2: Resurrectedor even the original version, players are tasked with the impossible: to single-handedly defeat the three Prime Evils because Tyrael's too lazy and everyone else in Sanctuary is either dysfunctional, drunk, or dying. The best builds for the Ultimate Evil Edition on the PlayStation 3 or Xbox 360 are found in the Patch 2.0 Data Archive. I sometimes get 1-2, or even in rare cases zero legendaries from a GR35 clear, plus the rifts are so fast that you spend a significant amount of time waiting for the rift timer. Deaths Breaths: 278 Forgotten Souls: 48 Greater Rift Keys: 13. Of course the best option is fast farming T10 with Sages Set + your normal. I still think T6 full clears are the best for Forgotten Souls. This guide shows the best methods to gain massive amounts of EXP, Cursed Chests, Gems, and Legendary Items through farming. This data is from level 70 Wizards playing the Reaper of Souls expansion on PC or Mac, and also matches the current patch for the Diablo III Ultimate Evil Edition on PlayStation 4 and Xbox One. We've done some speed GRs (35 in an average of 2.5 minutes, everything between 1:40 and 4:20 to completion).If you'd go to act 4 inferno, the same quality of gems would drop, but the pace at which you could kill enemies would very likely be much slower. In act 1 inferno, flawless squared gems are dropped, and those are the highest gems that can be dropped. All hardcore Wizards regardless of elite monster kills. In my opinion, the best place to farm gems quickly is act 1 inferno.By following the tips provided, you can increase your chances of obtaining the desired outcome. 90s can still give yellows while 100s can only drop leggos/set items. Farming for forgotten souls in Diablo 3 can be a quick and easy process. The softcore Wizards who are in the top 25% of elite monster kills. Nothing is faster then GR spam for them and item drop caps at 90 so fastest you can do up to 90 will give you best drops.The softcore Wizards who are in the bottom 75% of elite monster kills.Each set of data contains charts of three groups of Wizards:. Experience Farming is the best way to get a constant and reliable power increase in this game. Arcane Dust, Veiled Crystals, and Forgotten Souls for white, blue, yellow. The first of these is a way for you, as an classes with no resources whatsoever and limited abilities at their. There are many different Classes with their own individual builds in Diablo III. That includes farming Ancient Legendary items, running Nephalem Rifts and Greater Rifts in Adventure Mode, or quickly gaining Paragon Levels. A guide on how to farm reusable parts as fast as possible in Diablo 3 Reaper. Click it and it will return to town and can be used indefinitely on non-Season characters. The skills and runes in these charts are based on the choices of level 70 Wizards using the best Wizard builds for end-game content. Make your way through the Elder Sanctum until you find it.Gambling after should net you another 3-6 legendaries. They are calculated from data gathered on more than 6 million Diablo III characters. By the end of your 3rd or 4th run, youll have a full inventory of trash legendary and set items you can break down for souls. These are the most popular Diablo III Wizard builds and skills used by level 70 Wizards in Diablo III PC Patch 2.3, in the Diablo III: Reaper of Souls expansion. 7 - 9 Moreover, data from 1 study 10 found a large variation in trauma activation fees at trauma centers in Florida, with for-profit hospitals charging substantially more. 6 While a trauma activation fee may be justified, popular media articles have decried the large amounts charged for trauma activation for, in some cases, very minor injuries. Trauma activation fees were first approved in 2002 after it was argued that the high cost of running trauma centers threatened to shut down many essential trauma centers. 5 These patients or their insurance payers may thus bear the monetary expense of a visit to a trauma center without having a medical need for it.Īmong the charges that may be accrued for a visit to a trauma center is the trauma activation fee, which is a charge for the mobilization of the medical professionals who make up the trauma team. 3, 4 At the same time, previous data have suggested that more than one-third of patients brought to trauma centers do not meet field triage guidelines for transport to trauma centers. 2 However, trauma centers have been found to improve survival rates among patients with serious injuries. 1 Patients with serious traumatic injuries often receive care at trauma centers where the cost is high, at least in part because trauma centers must be continuously prepared to treat life-threatening injuries. Traumatic injuries are a major contributor to total US health care spending. Therefore, standardization of trauma activation fees is warranted. These findings suggest that some patients with serious traumatic injuries will incur disproportionately high trauma activation fees depending on the trauma center to which they are brought. In addition, for-profit hospitals charged more than other types of hospitals. Regional variation in these fees was substantial, with hospitals in the West charging substantially more than those in other locations. Trauma activation fees were also higher at for-profit hospitals, most of which were owned by the HCA Healthcare system, which had 43 trauma centers and a median (IQR) tier 1 trauma activation fee of $29 999 ($20 196-$37 589).Ĭonclusions And Relevance In this study, trauma activation fees varied widely among hospitals in the US. Median (IQR) trauma activation fees were highest in the West ($18 099 ), especially in California, where the median (IQR) activation fee was $24 057 ($15 979-$33 618). Overall, the median (IQR) tier 1 trauma activation fee was $9500 ($5601-$17 805), and the mean (SD) tier 1 trauma activation fee was $13 349 ($11 034) these fees ranged from $1000 to $61 734. There were 176 adult level I trauma centers and 200 adult level II trauma centers 69 centers had for-profit status, and 415 were academic. Results Of 523 trauma centers included in the analysis, most were located in the Midwest (180 centers) and West (129 centers). Main Outcomes and Measures Median and mean trauma activation fees nationally and stratified by location, hospital system, and other hospital characteristics. Linear regression analyses were performed to assess potential associations between hospital characteristics (type of control, hospital system, number of staffed beds, and academic vs nonacademic status) and trauma activation fees. All data were collected between January 2 and March 11, 2022. Hospital-specific data were obtained from the American Hospital Association website. Two levels of trauma activation fees were recorded: tier 1 (full activation) and tier 2 (partial activation). Each hospital’s publicly available chargemaster (a comprehensive list of a hospital’s products, procedures, and services) was searched to obtain its trauma activation fees. Five military hospitals were excluded, and trauma activation fees could not be found for 18 trauma centers the remaining 523 hospitals were included in the analysis. Objective To evaluate the variability of trauma activation fees from trauma centers across the US and examine whether certain hospital characteristics are associated with higher activation fees.ĭesign, Setting, and Participants This cross-sectional study used data from the American College of Surgeons website to identify all trauma centers in the US that were listed as verified from inception of the verification database through Ma(N = 546). Regional and hospital-related variations in trauma activation fees across the US have not been formally assessed. Importance Trauma centers must be readily equipped to handle a variety of life-threatening injuries and consequently may charge a fee for the activation of their trauma team. Shared Decision Making and Communication.Scientific Discovery and the Future of Medicine.Health Care Economics, Insurance, Payment.Clinical Implications of Basic Neuroscience.Challenges in Clinical Electrocardiography. If you miss the days of clanky keyboards, or you’d like an audible indication when your keyboard’s keys are recognized, this little utility may interest you. The mobile version for Android and iOS will be out soon. and currently supports the following platforms -Windows, Mac, Linux, 64-bit edition. You can download it here from its homepage. It’s open-source, completely free to use. However, additional sound sets are coming out, soon. Again pressing the button changes the behavior and allows you to listen to the sound.Īll in all, it’s a handy app, but it has one shortcoming is that users have no option to change the default sound. Lastly, there’s a ‘ Pause’ button that stops the sound when you hit it. You can choose any number between these limits by simply sliding the bar to the desired level. The minimum sound limit is 0 and the maximum sound limit is 100. There’s a volume slider that allows you to set the volume of the sound. We would like the maker of the app to add some more options in the future. Also, that’s the only sound currently available. Its interface appears quite simple with only a few options.īy default, the app is set to CherryMXBrown – PBT Keycaps. When you download the app and run it, the main window of the app shows up. The app is large in size to some extent, measuring about 44.5 MB in size. If you are looking for a way to make your keyboard make sounds when you type in Windows 10 then you are sure to like Mechvibes. Play Mechanical Keyboard Sounds as you type on keyboard 1] Mechvibes It’s more like a membrane or mech key with any kind of switch. They let you define and play mechanical keypress sound as you type your current keyboard. Also, the sound from keyboard key presses is known to irritate some – especially those who suffer from Misophonia. Both are free apps that let you play mechanical keyboard sounds as you type on your computer keyboard. If you were a fan of the mechanical keyboard sounds and would like to experience them on your PC keyboard – try Mechvibes or ClicKey. *There have been reported incidences where if you don't approach the painting after it has been painted, Dingo won't think it has been painted. Get the confusion grenade merit badge and use it to temporarily stop the bull so the dog can paint the poster on the wall, approach the painting* then return to Dingo Inflagrante for your reward. If you're trying to achieve 100 completion in Psychonauts 2, you'll need to complete the Making Peace achievement, however, the instructions aren't all that clear and simply require you to. □Black Velvetopia #2 - THIS ONE CAN BE MISSED if you complete Black Velvetopia. « La Silicon Valley est le microcosme des excès du capitalisme »,CHRONIQUE.The event will take place on June 3 rd, from 11am to 8pm in Los Angeles, California. Zach Leary will present the tapes, and share personal stories, provide context, moderate an open discussion and answer as many questions as he can. Psychonauts 2 is the type of game that is begging to be completed fully. We've got tons of guides to help you do that, but now we're deep-diving into the brains of achievement hunters and platinum enthusiasts with this complete accolade guide. Black Velvetopia #1 - Hang the window painting on the upper ledge at end of the of an alley near the Queen of Diamond entrance, enter the newly created window. You will have the opportunity to learn about Leary’s life and theories, in his own words. Psychonauts 2 is the type of game that is begging to be completed fully. Beginner’s Guide: 32 Essential Tips and Tricks for Getting Started in Psychonauts 2.Waterloo World - On the pink bridge near the peasant who is unarmed. There are relatively few pieces of Emotional Luggage in Psychonauts 2, with most of them hidden away in secret areas, dark corners, or high up in the air.The Milkman Conspiracy - You need telephone access, it's on the island accessible from the telephone pole after the road crew.Lungfishopolis - on top of a skyscraper after the monorail tunnel.Brain Tumbler Experiment - (after Sasha's Shooting Gallery) behind the thorny tower. I'm Gonna Live Forever Find all Golden HelmetsĪll but one can be retrieved at any time in the following areas: I also think you get rid of some of the adds that pop up on almost every level. I just started this app a phew days ago and I’m already passed level 200. Also I think the levels could be a little harder. It could really help the players of this game with future levels. It makes the game a lot easier and all but I think it would be a lot cooler if you could show us how we could have passed the level. Another thing is the skip this level thing. I think you should work on that a little bit. It doesn’t really bother me but when I showed this game to my friends, they got really annoyed and deleted the game. so for starters you guys made the game where it repeats levels. Hey developer(s)□□! I love the game! It’s really fun and really addictive! I just have a phew things that I think could improve the game. Me and my friend hv been seeing who can pass faster but do get board after about 15-20 min so that is why I give it 3 stars and guys keep on going it gets better This is my new favorite game to play whale on the phone because it doesn’t make much noise and is relaxing. As I explained before it keeps you from getting board and doesn’t take awY from things. Ok so I thought I was a little harsh I give this 3 ✨. I didn’t delete the app because it takes up time when you are absolutely board out of your Skull!! Or when you want to play a game when you are watching tv but not take away from the show over all I would hv prolly have given it a 0 if it was not at first fun and helps you from not losing your mind when you are board. Then like ever 10-15 levels the same levels were repeating and we are both pretty good at this game so 10-15 levels was like 1-2 min tops. We knew the levels repeated but it was never that close I mean come on. Not even 10 levels later it repeated again me and my friend were both playing and started to get mad and frustrated. When you go back to it the next day or later you start to realize the levels repeat especially in the 200’s I had a super hard level and a passed it. At first you get super addicted to the game and can’t stop playing and you think it is the best but it is not. Ok so if you hv read any of these reviews you would see that ever one says the levels repeat. Install Roller Splat! on your Mac using the same steps for Windows OS above. Once Roller Splat! is downloaded inside the emulator, locate/click the "All apps" icon to access a page containing all your installed applications including Roller Splat!.The search will reveal the Roller Splat! app icon.Open the Emulator app you installed » goto its search bar and search "Roller Splat!".On your computer, goto the Downloads folder » click to install Bluestacks.exe or Nox.exe » Accept the License Agreements » Follow the on-screen prompts to complete installation. Install the emulator on your PC or Mac:.Download Bluestacks Pc or Mac software Here >. We recommend Bluestacks because you can easily find solutions online if you run into problems while using it. Download an Android emulator for PC and Mac:. The emulsion PCR protocol based on ABIL EM 90 surfactant was adapted from 20, with revisions to allow for single-stage, cell-based RT and amplification reactions. We employed a strategy of starting from RNA, which benefits from a high abundance of molecules encoding TCR alpha and beta chains and universal primer annealing sites on the constant regions of the TCR chains 18, 19. (D) Data shown are representative of three experiments performed. PCR suppression (lanes 4–6) eliminated random overlap extension efficiently compared to reactions performed without such suppression (lanes 1–3). (D) Postemulsion amplification of fused TCR molecules using: (lanes 1, 4) product of emulsion reaction that initially contained all primers from the start (αβ), (lanes 2, 5) combined products of two separate emulsions, each initially containing only primers for either TCR alpha or beta amplification (as a control for random overlap extension after emulsion), and (lanes 3, 6) combined product of two separate emulsions that initially contained primers for either alpha or beta TCR amplification but were joined after RT step (as a control for random overlap extension due to fusion of cell-containing emulsion droplets during PCR). (C) Reaction products are extracted from the emulsion and fused molecules of interest are selectively amplified, while nonfused molecules are suppressed by blockers. (B) Released TCR alpha and beta mRNAs are reverse-transcribed at 50☌ with specific primers, amplified, and overlap extended within each droplet. 1A).Ĭell-based emulsion RT-PCR technique for identifying TCR alpha–beta chain pairing. The key requirement for the success of our approach is the ability to generate a representative library of TCR alpha and beta genes that are fused within emulsion reactions, wherein individual T cells are each contained within a separate minute droplet of sufficient reaction volume (Fig. Results and discussion RT and amplification from cells in emulsion Notably, the whole technology is simple to implement and does not require any special equipment. We overcame the primary obstacle of random, nonspecific overlap extension during postemulsion amplification reactions with a new PCR-suppression technique that we invented to specifically and efficiently block the 3′-ends of the free, nonoverlapped alpha and beta chain PCR products. Here, we report a new approach for identifying TCR alpha–beta pairing, based on specific RT of TCR alpha and beta chain mRNA, PCR amplification, and subsequent coupling via overlap extension 17, all performed within emulsion droplets each of which contains a single T cell. However, this assay is quite laborious and requires the fabrication of custom chips, while its efficiency is not fully clear. Recently, a micro-well plate assay was reported to pair human immunoglobulin heavy and light chains for high-throughput sequencing 16. Furthermore, those approaches that could yield massive output based on the amplification and assembly of genes in fixed cells 14, 15 have not demonstrated feasibility for the analysis of complex samples. Phage and yeast display technologies 11- 13, although efficient for isolation of antigen-specific antibodies, rely on random pairing and do not provide information on the native pairs of chains. While these approaches are feasible to identify native chain pairs, they can only be performed serially for a limited number of clones, thereby prohibiting comprehensive analysis of most biological samples. Native pairs of TCR or antibody chains can also be identified after culturing of lymphocyte clones 5, or sorting of narrow antigen-specific populations of T cells 9 or B cells 10. Because of this, methods for efficient identification of their functional units - native pairs of heavy/light antibody or alpha/beta TCR chains - have been highly desired since decades.Ī series of different approaches have been developed to this end, including hybrid cells 4, single-cell PCR 5- 7, and frequency-based pairing 8. Analysis of the native TCR and antibody repertoires is also of fundamental importance for our understanding of adaptive immunity in health and disease 3. Antibodies and T-cell receptors (TCRs), the weapons of adaptive immunity capable of selective recognition of specific antigens, represent an invaluable resource for biological studies and medical applications 1, 2. |